Phytochemistry (extraction, identification and measurement of active components)
L. Moradipour; V. Payam Noor
Abstract
In biotechnology processes, increasing the production of biomass and secondary metabolites is one of the economic factors of such projects. The present study was conducted to investigate the effects of magnetic field as an abiotic elicitor on increase of biomass and secondary metabolites of Ganoderma ...
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In biotechnology processes, increasing the production of biomass and secondary metabolites is one of the economic factors of such projects. The present study was conducted to investigate the effects of magnetic field as an abiotic elicitor on increase of biomass and secondary metabolites of Ganoderma lucidum. Mycelium with a diameter of 1 cm of pured fungi was exposed to magnetism at four levels including 0, 20, 40, and 60 mT for 0, 30, 60, and 90 min with three replications. The best treatment was selected based on growth rate and antioxidant activity. The amounts of secondary compounds in mycelium under selected magnetic field (60 mT for 90 min) including betulin, betulinic acid, ascorbic acid, astaxanthin, total polysaccharide, and antioxidants were measured and compared with control and natural fungi. The results showed that magnetization increased betulin (a potent anticancer triterpene) more than 2.5 times compared to natural fungi and 3.7 times compared to control mycelium. Polysaccharides concentration in the treated mycelium and control media was obtained 5.05 and 5.17 times more than natural fungi, respectively. Ganoderic acid content was measured in the treated mycelium (intracellular) and PDB medium (extracellular). The highest amount of ganoderic acid (1.9 mg.ml-1) was obtained in the treated mycelium. Natural fungi and PBD medium had almost the same amount of this compound (1.87 and 1.86 mg.ml-1). The amount of betulinic acid (an anticancer compound derived from betulin), ascorbic acid, and astaxanthin was obtained high in natural fungus followed by 90 min magnetization at 60mT level treatment and control, respectively. Antioxidants percentage was about 85% in control and about 75% in natural fungi and treated mycelium. According to the results, there seems to be a good prospect to use in vitro cultures of Ganoderma instead of using natural fungi and also constructing costly farms.
